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KMID : 0644120050180040085
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Journal of Oriental Gynecology
2005 Volume.18 No. 4 p.85 ~ p.105
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Effects of Chungganhaewooltang on Serum Levels of Histamine and Corticosterone and Immune Response after Immobilization-Stress or Cold-Stress in Mice
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Jung Woo-Suk
Kim Song-Baeg
Yoo Sim-Keun
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Abstract
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Purpose : Investigate the effects of Chungganhaewooltang(CHT) on immobilization-stress or cold-stress in C576BL/6J mice.
Methods : Male C57BL/6J 30 mice of weighting 18{pm}2g, were divided into sixs groups including the immobilization-stress group(5heads), after immobilization-stress CHT oral administration(500mg/kg) groups(5heads), cold-stress group(5heads) and after cold-stress CHT oral administration(500mg/kg) groups(5heads). then we observed changes in the serum histamine and corticosterone level and changes immune system
Results : Immobilization-stress or cold-stress increased the serum level of histamine and corticosterone. CHT decreased the serum level of histamine and corticosterone increased by cold-stress. CHT inhibited the release of histamine from mast cells at the concentration of 0.1 mg/ml. In addition, immobilization-stress or cold-stress decreased the cell viability of murine thymocytes and splenocytes. CHT increased the cell viability of thymocytes decreased by immobilization-stress or cold-stress, but did not affect the cell viability of splenocytes decreased by immobilization-stress or cold-stress. Also immobilization-stress or cold-stress increased DNA fragmentation of thymocytes and splenocytes. CHT decreased DNA fragmentation of thymocytes increased by immobilization-stress or cold-stress, but did not affect DNA fragmentation of splenocytes increased by immobilization-stress or cold-stress. Immobilization-stress increased the population of thymic CD4^+ cells. CHT decreased the population of thymic CD4^+ cells increased by immobolization-stress. Immobilization-stress or cold-stress decreased the population of B220^+ cells and increased the population of thy1^+ cells. CHT decreased the population of thy1^+ cells increased by immobilization-stress or cold-stress. Immobilization-stress or cold-stress increased the population of splenic CD4^+ cells and CD8^+ cells. CHT decreased the population of splenic CD4^+ cells increased by immobolization-stress or cold-stress. Immobilization-stress or cold-stress decreased the production of {gamma}-interferon(IFN) interleukin(IL)-2 and IL-4. CHT enhanced the production of {gamma}-IFN decreased by immobilization-stress or cold-stress but did not affect the production of IL-2 and IL-4 decreased by immobilization-stress or cold-stress. Furthermore, Immobilization- stress or cold-stress decreased the phagocytic activity of peritoneal macrophages and the production of nitric oxide. CHT enhanced the phagocytic activity and nitric oxide production decreased by cold-stress.
Conclusion : CHT may be useful for the prevention and treatment of stress via suppression of serum histamine and corticosterone level and enhancement of immune response.
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KEYWORD
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Chungganhaewooltang(CHT), immobilization-stress, cold-stress, histamine, corticosterone, immune response
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